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Updated: Jul 8, 2025

14:36
Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
Published on: August 26, 2009
11.2K
Nanoscale photobiotinylation, pulldown and sequencing of region-specific DNA from intact cells
Thomas C Roberts1, Max Kushner2, Jack C Crowley3
1Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY USA 14853.
Biorxiv : the Preprint Server for Biology
|December 18, 2023
Abstract:
Femto-seq is a novel nanoscale optical method that can be used to obtain DNA sequence information from targeted regions around a specific locus or other nuclear regions of interest. Two-photon excitation is used to photobiotinylate femtoliter volumes of chromatin within the nucleus, allowing for subsequent isolation and sequencing of DNA, and bioinformatic mapping of any nuclear region of interest in a select set of cells from a heterogenous population.

