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Intelligent Electrochemical Point-of-Care Test Method with Interface Control Based on DNA Pyramids: Aflatoxin B1
Wenqin Wu1, Yizhen Bai1, Tiantian Zhao1
1Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Key Laboratory of Detection for Mycotoxins, National Reference Laboratory for Agricultural Testing (Biotoxin), Hubei Hongshan Lab, Wuhan 430062, China.
A novel electrochemical sensor using DNA pyramids enables sensitive detection of aflatoxin B1 (AFB1) at the point-of-care. This intelligent test offers accurate results in complex samples, aiding food and environmental safety.
Area of Science:
- Biosensors
- Nanotechnology
- Analytical Chemistry
Background:
- Sensitive detection of small molecules like aflatoxin B1 (AFB1) is crucial for food and environmental safety.
- Existing point-of-care tests (POCT) face challenges in surface control for enhanced sensitivity.
- Developing intelligent POCT (iPOCT) methods is essential for rapid and reliable analysis.
Purpose of the Study:
- To develop an electrochemical sensor for sensitive and intelligent detection of AFB1.
- To utilize DNA pyramids (DNP) for improved surface assembly and recognition probe orientation.
- To integrate the sensor with a smartphone and mobile electrochemical workstation for iPOCT.
Main Methods:
- Fabrication of an electrochemical sensor utilizing DNA pyramids (DNP) for controlled probe immobilization.
- Combination of the DNP-based biosensor with electrochemical (Ec) iPOCT for AFB1 detection.
- Utilizing a smartphone and app for intelligent data acquisition and analysis.
Main Results:
- Achieved a low detection limit of 3 pg/mL for AFB1 with a linear range of 0.006-30 ng/mL.
- Demonstrated high accuracy (82.15-100.53% recovery) in complex matrices like peanut, soybean, corn, and lake water.
- Validated results against high-performance liquid chromatography-tandem mass spectrometry, showing consistent performance.
Conclusions:
- The developed DNP-based electrochemical iPOCT offers a sensitive, selective, and reproducible method for AFB1 detection.
- The DNP structure enhances antigen-antibody binding efficiency and electrochemical signal transduction.
- This technology provides a promising platform for on-site monitoring of AFB1 in food and environmental samples.
