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Advances in Cryopreservatives: Exploring Safer Alternatives
Laura Lomba1, Cristina B García1, Lucía Benito1
1Facultad de Ciencias de la Salud, Universidad San Jorge. Campus Universitario, Autov A23 km 299, 50830 Villanueva de Gállego, Zaragoza, Spain.
ACS Biomaterials Science & Engineering
|December 23, 2023
Summary
This review explores cryopreservation substances, highlighting traditional options like alcohols and sugars, and newer, less toxic alternatives such as ionic liquids and deep eutectic solvents for cell and tissue preservation.
Area of Science:
- Biomedical science and research
- Cellular and tissue preservation technologies
Background:
- Cryopreservation is essential in biomedical research for preserving cells, tissues, and organs.
- Conventional cryoprotectants like dimethyl sulfoxide (DMSO) pose toxicity challenges for sensitive samples.
Purpose of the Study:
- To review diverse cryopreservation substances.
- To highlight emerging, less toxic alternatives for improved sample viability.
Main Methods:
- Literature review of cryopreservation agents.
- Categorization of cryoprotectants based on chemical families.
- Analysis of toxicity and efficacy of different cryoprotective compounds.
Main Results:
- Established cryoprotectants include alcohols, sugars, and sulfoxides.
- Novel compounds like ionic liquids, deep eutectic solvents, and polymers show promise.
- These newer agents offer reduced toxicity compared to traditional options.
Conclusions:
- A variety of cryopreservation agents are available, each with specific applications and limitations.
- Emerging cryoprotectants present opportunities for developing safer and more effective preservation methods.
- Further research into novel compounds can advance the field of cryobiology.
