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Ordered mesoporous silicas for potential applications in solid vaccine formulations.
Matheus C R Miranda1, Carmen M Nunes1, Luana F Santos1
1Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema, SP, Brazil.
Vaccine
|December 25, 2023
Summary
Ordered mesoporous silica (SBA-15) effectively carries antigens for vaccine development. Freeze-drying and evaporation methods preserve antigen structure and enhance immunogenicity, making SBA-15 a promising antigen carrier.
Area of Science:
- Materials Science
- Biotechnology
- Nanotechnology
Background:
- Ordered mesoporous silica (SBA-15) offers high surface area and controlled porosity, making it suitable for antigen encapsulation.
- Developing efficient vaccine formulations requires carriers that protect antigens and enhance their immunogenicity.
Purpose of the Study:
- To investigate the impact of freeze-drying and evaporation on the physicochemical properties and immunogenicity of BSA-SBA-15 complexes.
- To evaluate SBA-15 as a carrier for enhancing antigen immunogenicity.
Main Methods:
- Synthesis of BSA-SBA-15 complexes.
- Characterization using Small Angle X-ray Scattering, Nitrogen Adsorption Isotherm, Fluorescence Spectroscopy, and Circular Dichroism.
- Immunogenic analysis via anti-BSA IgG detection.
Main Results:
- SBA-15 structure was preserved post-BSA incorporation, with BSA aggregating in macropores.
- Surface area and pore volume decreased, confirming BSA encapsulation.
- BSA's native structure and aromatic residue environment were maintained.
- SBA-15 significantly increased BSA immunogenicity (three-fold higher than BSA alone), irrespective of drying method.
Conclusions:
- Freeze-drying and evaporation are suitable methods for preparing BSA-SBA-15 immunogenic complexes.
- SBA-15 enhances the immunogenic activity of encapsulated antigens.
- SBA-15 is a promising material for developing advanced vaccine formulations.
