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Preparation and application of caffeic acid imprinted polymer
Şeyda Karaman Ersoy1, Esma Tütem2, Kevser Sözgen Başkan2
1Division of Analytical Chemistry, Faculty of Pharmacy, Fenerbahçe University, İstanbul, Turkiye.
Turkish Journal of Chemistry
|January 4, 2024
Summary
Molecularly imprinted polymers were developed for extracting caffeic acid (CA) and chlorogenic acid (CLA). The optimized polymer demonstrated effective recognition and separation capabilities from complex samples.
Area of Science:
- Polymer Chemistry
- Analytical Chemistry
- Biochemistry
Background:
- Caffeic acid (CA) and chlorogenic acid (CLA) are important phenolic compounds found in various natural products.
- Efficient extraction and purification methods are crucial for their analysis in complex matrices.
- Molecular imprinting offers a selective recognition approach for target analytes.
Purpose of the Study:
- To synthesize and characterize molecularly imprinted polymers (MIPs) for selective extraction of CA.
- To evaluate the performance of MIPs for the simultaneous extraction of CA and CLA.
- To optimize the molecular imprinted solid phase extraction (MISPE) method for CA and CLA purification.
Main Methods:
- Synthesis of MIPs using caffeic acid as a template and various monomers (4-vinyl pyridine, methacrylic acid, acrylamide, 1-vinyl imidazole) and crosslinker (ethylene glycol dimethacrylate).
- Preparation of nonimprinted polymers (NIPs) for control experiments.
- Adsorption studies (dynamic and static) to assess polymer binding characteristics.
- Optimization of MISPE protocol (loading, washing, elution) using High-Performance Liquid Chromatography (HPLC) analysis.
Main Results:
- The optimal MIP formulation was identified with a 1:4:16 mole ratio of caffeic acid:4-vinylpyridine:ethylene glycol dimethacrylate.
- MIPs exhibited selective adsorption properties for CA compared to NIPs.
- The optimized MISPE method successfully separated and purified CA and CLA from complex samples, validated by HPLC.
Conclusions:
- Molecularly imprinted polymers provide a highly selective and efficient tool for the extraction and purification of caffeic acid and chlorogenic acid.
- The developed MISPE method is a robust technique for analyzing these phenolic compounds in complex natural product matrices.
- This study highlights the potential of tailored MIPs in analytical chemistry for targeted analyte isolation.
Keywords:
Caffeic acid imprinted polymercaffeic acid recoverychlorogenic acid recoverygreen coffee beanmolecularly imprinted solid phase extractionMore Related Videos
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