Related Experiment Video
Updated: Jun 9, 2026
Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY
Published on: June 30, 2016
Optical clearing agents based on metallic and dielectric nanoparticles for caries diagnostic by optical coherence
Vanda Sanderana Macedo Carneiro1, Eloiza Leonardo de Melo2, Claudia Cristina Brainer de Oliveira Mota3,4
1Graduate Program in Dentistry, Universidade de Pernambuco (PPGO-UPE), Recife, Brazil.
Objective:
This study aimed to assess the effect of titanium dioxide (TiO2) and silver (Ag) nanoparticles dispersed in glycerol or water, serving as optical clearing agents nanocolloids (OCAs-NC), for improving optical coherence tomography (OCT) images and highlighting incipient lesions in ex vivo human teeth.
Materials And Methods:
Twelve human teeth with incipient lesions were divided into seven groups according to the OCA-NC; they were subjected to G1 (air), G2 (glycerol), G3 (TiO2 0.1%), G4 (TiO2 0.01%), G5 (TiO2 0.001%), G6 (AgNO3 10%), and G7 (AgNO3 100%). The OCA-NC was applied to the occlusal surface, and two-dimensional images of the specimens were analyzed using OCT (930 nm central wavelength; 100 nm bandwidth; 5 mW output power; axial resolution of 7/5.3 μm in water and air, respectively; lateral resolution of 8 μm; and light penetration depth of 1.6 mm inside the sample).
Results:
The findings demonstrated that the utilization of OCAs-NC containing metallic or dielectric nanoparticles (AgNO3 and TiO2) led to improved differentiation between sound and demineralized enamel on occlusal surfaces. Additionally, it enhanced the depth of image penetration when analyzing this hard tissue with OCT.
Clinical Relevance:
In the current context of minimally invasive dentistry, the use of OCAs-NC in conjunction with OCT can provide clinicians with early diagnosis, allowing for the determination of less/more invasive therapies and consequently halting the disease before cavitation of dental tissues occurs.
