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Patch-seq: Multimodal Profiling of Single-Cell Morphology, Electrophysiology, and Gene Expression
Cathryn R Cadwell1, Andreas S Tolias2
1Departments of Neurological Surgery and Pathology, School of Medicine, University of California San Francisco, San Francisco, CA, USA. Cathryn.Cadwell@ucsf.edu.
Methods in Molecular Biology (Clifton, N.J.)
|January 9, 2024
Summary
Patch-seq is a new technique that allows researchers to study single cells comprehensively. This method links cell morphology, electrophysiology, and gene expression for deeper biological insights.
Area of Science:
- Cell biology
- Neuroscience
- Genomics
Background:
- Understanding single-cell heterogeneity is crucial but limited by profiling techniques.
- Multimodal profiling of individual cells remains a significant challenge in biological research.
Purpose of the Study:
- To present a detailed protocol for the Patch-seq technique.
- To enable comprehensive single-cell analysis by integrating morphology, electrophysiology, and transcriptomics.
Main Methods:
- The study details the Patch-seq technique, combining whole-cell patch clamp recording, immunohistochemistry, and single-cell RNA-sequencing (scRNA-seq).
- The protocol focuses on obtaining high-quality multimodal data from individual cells.
Main Results:
- The Patch-seq protocol yields high-quality morphological, electrophysiological, and transcriptomic data.
- This technique allows for the exploration of multidimensional phenotypic variability within cell populations.
Conclusions:
- Patch-seq provides a powerful tool for correlating gene expression with cellular phenotype at the single-cell level.
- This method facilitates a deeper understanding of cell diversity and function.
