Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Label-Free Dual-Mode Flow Cytometry for Single-Cell Physicochemical Property Analysis.

Analytical chemistry·2026
Same author

Dual-Branch Fusion Network: Precise Decoding of Lower Limb Multi-Joint Torque.

IEEE transactions on bio-medical engineering·2026
Same author

Atomic-scale mechanism unlocks thermal-stable high-κ performance in HfO<sub>2</sub> via coherent interfaces.

Nature communications·2026
Same author

Deficiency of extracellular vesicles miR-32 from bone marrow mesenchymal stem cells alleviates vascular calcification in type 2 diabetes by inhibiting endothelial ferroptosis.

Stem cell research & therapy·2026
Same author

Treadmill exercise alleviates Alzheimer's disease pathologies in APP/PS1 mice through modulation of microglial glucose metabolic reprogramming.

Frontiers in aging neuroscience·2026
Same author

Network pharmacology analysis and in vitro experimental validation of liriodenine against non-small cell lung cancer.

BMC cancer·2025

Related Experiment Video

Updated: Jul 5, 2025

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

27.5K

Capillarity Enabled Large-Array Liquid Metal Electrodes for Compact and High-Throughput Dielectrophoretic

Huichao Chai1, Junwen Zhu1, Yongxiang Feng1

  • 1State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instrument, Tsinghua University, Beijing, 100084, P. R. China.

Advanced Materials (Deerfield Beach, Fla.)
|January 18, 2024
PubMed
Summary

This study introduces a novel method for high-density liquid metal alloy (LMA) electrode arrays in microfluidic devices, significantly boosting dielectrophoresis (DEP) particle separation throughput and enabling efficient cell separation.

Keywords:
capillaritydielectrophoresislarge‐array electrodesliquid metalmicrofluidics

More Related Videos

A Method to Manipulate Surface Tension of a Liquid Metal via Surface Oxidation and Reduction
09:20

A Method to Manipulate Surface Tension of a Liquid Metal via Surface Oxidation and Reduction

Published on: January 26, 2016

15.4K
Development of a 3D Graphene Electrode Dielectrophoretic Device
11:15

Development of a 3D Graphene Electrode Dielectrophoretic Device

Published on: June 22, 2014

12.0K

Related Experiment Videos

Last Updated: Jul 5, 2025

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

27.5K
A Method to Manipulate Surface Tension of a Liquid Metal via Surface Oxidation and Reduction
09:20

A Method to Manipulate Surface Tension of a Liquid Metal via Surface Oxidation and Reduction

Published on: January 26, 2016

15.4K
Development of a 3D Graphene Electrode Dielectrophoretic Device
11:15

Development of a 3D Graphene Electrode Dielectrophoretic Device

Published on: June 22, 2014

12.0K

Area of Science:

  • Microfluidics
  • Biotechnology
  • Materials Science

Background:

  • Dielectrophoresis (DEP) particle separation offers label-free, controlled, and low-damage advantages.
  • Liquid metal alloy (LMA) sidewall microelectrodes provide strong DEP forces due to their thickness.
  • Current LMA devices struggle with integrating large electrode arrays in small spaces, limiting flow rates and throughput.

Purpose of the Study:

  • To develop a facile and versatile method for integrating high-density thick LMA electrodes in microfluidic devices.
  • To enhance DEP particle separation throughput by utilizing an arrayed electrode configuration.
  • To demonstrate the efficacy of the developed platform for complex biological sample separation.

Main Methods:

  • Co-designing microfluidic channels with capillary burst valves (CBVs) with specific burst pressures.
  • Facilitating self-assembly of LMA electrode arrays via simple hand-push injection.
  • Fabricating a compact chip with 5000 pairs of sidewall electrodes for accumulative DEP deflection.

Main Results:

  • Achieved a tenfold increase in DEP deflection throughput compared to existing devices.
  • Successfully separated mixed biological samples, including human peripheral blood mononuclear cells and A549 cells.
  • Demonstrated high-throughput separation at a flow rate of 70 µL/min using the 5000-electrode-pair device.

Conclusions:

  • The proposed method enables efficient fabrication of LMA electrode arrays in microfluidic devices.
  • The developed platform offers a robust and versatile solution for high-throughput DEP separation applications.
  • This advancement can significantly facilitate the integration of LMA electrodes for various DEP-based applications.