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A FRET based ultrasensitive fluorescent aptasensor for 6'-sialyllactose detection
Jinri Chen1, Yuting Zhang2, Xiaoli Wang2
1The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China; Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, 59 Cangwu Road, Haizhou, 222005, China; State Key Laboratory of Genetic Engineering, MOE Engineering Research Centre of Gene Technology, School of Life Sciences, Fudan University, Shanghai, 200438, China.
A new fluorescence biosensor accurately detects 6'-sialyllactose (6'-SL), a key human milk oligosaccharide vital for infant development. This rapid method offers improved infant formula analysis and supports infant health monitoring.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Nanotechnology
Background:
- Human milk oligosaccharides like 6 -sialyllactose (6 -SL) are crucial for infant brain development and immunity.
- Accurate quantification of 6 -SL in infant formula is essential for nutritional quality control.
- Existing detection methods for 6 -SL lack efficiency and speed.
Purpose of the Study:
- To develop a rapid, sensitive, and specific biosensor for detecting 6 -SL.
- To utilize aptamers, catalytic hairpin assembly, and quantum dots for signal amplification.
Main Methods:
- A fluorescence biosensor was constructed using 6 -SL specific aptamers as recognition elements.
- Catalytic hairpin assembly (CHA) was employed for signal amplification.
- Quantum dots (QDs) were used as signal labels, coupled with fluorescence resonance energy transfer (FRET).
Main Results:
- The developed FRET-based biosensor achieved an ultra-sensitive detection limit of 0.3 nM for 6 -SL.
- The biosensor demonstrated a high signal-to-noise ratio, rapid detection times, and simplicity in operation.
- The method proved efficient and effective for detecting 6 -SL in actual samples.
Conclusions:
- This novel FRET-based fluorescent biosensor offers a highly efficient and sensitive platform for 6 -SL detection.
- The biosensor has significant potential for application in the quality control of infant formula and nutritional analysis.
- This advancement addresses the need for improved methods in analyzing crucial human milk oligosaccharides.
