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Antisolvent fabrication of monodisperse liposomes using novel ultrasonic microreactors: Process optimization,
Caihe Peng1, Xiaojing Zhu2, Jie Zhang2
1School of Pharmacy, Changchun University of Chinese Medicine, 130117 Changchun, China.
Ultrasonics Sonochemistry
|January 24, 2024
Summary
A novel ultrasonic microfluidic technology enables rapid, mass fabrication of uniform liposomes. This ultrasonic microreactor (USMR) system produces monodisperse liposomes with controllable sizes and narrow distribution, offering a breakthrough for nanomedicine production.
Area of Science:
- Biotechnology
- Materials Science
- Chemical Engineering
Background:
- Liposomes are crucial drug carriers, but their mass production with uniform size remains a significant challenge.
- Existing methods often struggle with scalability, reproducibility, and control over liposome characteristics.
Purpose of the Study:
- To develop a novel ultrasonic microfluidic technology for rapid and large-scale fabrication of monodisperse liposomes.
- To investigate the performance of two distinct ultrasonic microreactor (USMR) designs (co-flow and impinge flow) for liposome preparation.
Main Methods:
- Construction of two ultrasonic microreactor (USMR) configurations: USMR-CF (co-flow) and USMR-IF (impinge flow).
- Utilizing an antisolvent precipitation method within the USMRs to form liposomes.
- Systematic optimization of parameters including flow rate, ultrasonic power, and lipid concentration.
Main Results:
- Achieved monodisperse liposomes with tunable droplet sizes (60-100 nm) and polydispersity index (PDI) < 0.1.
- The USMR-IF demonstrated superior performance in reducing PDI and tuning droplet size compared to USMR-CF.
- Demonstrated high throughput (108 g/h) and stability with diverse lipid formulations, including HSPC-based liposomes (59.6 nm, PDI 0.08).
Conclusions:
- The novel ultrasonic microfluidic technology, particularly the USMR-IF, offers a rapid, fouling-free, and high-throughput method for fabricating monodisperse liposomes.
- This technology provides precise control over liposome size and distribution, paving the way for efficient nanomedicine production.
- The USMR system's stability and versatility make it a promising platform for manufacturing various nanomedicines.
