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Updated: Jul 4, 2025
Visualization of the Interstitial Cells of Cajal ICC Network in Mice
Published on: July 27, 2011
Protocol to visualize trans-interaction of clustered protocadherin using cIPAD, a fluorescent indicator, in cultured
Takashi Kanadome1, Natsumi Hoshino2, Takeharu Nagai3
1Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology (JST), Kawaguchi, Saitama 332-0012, Japan; Department of Biomolecular Science and Engineering, SANKEN (The Institute of Scientific and Industrial Research), Osaka University, 8-1 Mihogaoka, Ibaraki 567-0047, Japan.
Abstract:
cIPAD is a fluorescent indicator that allows the visualization of trans-interactions of clustered protocadherin (Pcdh), a cell adhesion molecule that mediates neuronal self-recognition. We describe steps for using HEK293T cells to visualize Pcdh trans-interactions across cells as a preliminary experiment before using dissociated mouse neurons. We then detail procedures for visualizing Pcdh trans-interactions between processes originating from the same neurons, which are considered as Pcdh-mediated neuronal self-recognition. For complete details on the use and execution of this protocol, please refer to Kanadome et al.1.
