Related Experiment Video
Updated: Jul 4, 2025
Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds
Published on: May 17, 2022
Orchid Micropropagation Using Temporary Immersion Systems: A Review
Obdulia Baltazar-Bernal1, Evelia Guadalupe Mora-González2, Marco A Ramírez-Mosqueda3
1Laboratório de Cultivo de Tejidos Vegetales, Colegio de Postgraduados Campus Cordoba, Amatlan de los Reyes, Veracruz, Mexico. obduliabb@colpos.mx.
Abstract:
Temporary immersion systems (TIS) have been used for orchid micropropagation. The main advantage of TIS use for micropropagation is that the explant is periodically immersed in nutrient media, and then, the nutrient solution is drained, which allows the explant tissue to stay in air. The current review resumes the application of TIS in orchid propagation. Fifty-three papers are discussed considering: explant, culture media, TIS bioreactor type, frequency and immersion time, and the TIS effects in acclimatization phase.
