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Plant Tissue Culture

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.

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Related Experiment Video

Updated: Jul 4, 2025

Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds

Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds

Published on: May 17, 2022

Orchid Micropropagation Using Temporary Immersion Systems: A Review.

Obdulia Baltazar-Bernal¹, Evelia Guadalupe Mora-González², Marco A Ramírez-Mosqueda³

¹Laboratório de Cultivo de Tejidos Vegetales, Colegio de Postgraduados Campus Cordoba, Amatlan de los Reyes, Veracruz, Mexico. obduliabb@colpos.mx.

Methods in Molecular Biology (Clifton, N.J.)

|January 29, 2024

Summary

This summary is machine-generated.

Temporary immersion systems (TIS) offer an efficient method for orchid micropropagation by periodically exposing explants to nutrient media and air. This review analyzes TIS applications in orchid propagation, covering key factors and acclimatization effects.

Keywords:

Immersion frequency Large-scale production Orchidaceae PLBs Wild orchid

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Last Updated: Jul 4, 2025

Microscopy Techniques for Interpreting Fungal Colonization in Mycoheterotrophic Plants Tissues and Symbiotic Germination of Seeds

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Published on: May 22, 2018

Area of Science:

Plant Biotechnology
Horticultural Science
Plant Tissue Culture

Background:

Orchid micropropagation faces challenges in achieving efficient and scalable plantlet production.
Temporary immersion systems (TIS) provide a promising alternative to conventional static culture methods.

Conclusions:

Temporary immersion systems are a valuable tool for improving orchid micropropagation efficiency.
Further research optimizing TIS parameters for diverse orchid species is warranted.
TIS facilitates better acclimatization, potentially reducing losses in ex vitro cultivation.