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Plant Tissue Culture

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.

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Updated: Apr 17, 2026

Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples

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Published on: November 23, 2016

In Vitro Storage Techniques for Orchids Conservation: A Review.

Evelia Guadalupe Mora-González¹, Obdulia Baltazar-Bernal², Marco A Ramírez-Mosqueda³

¹Universidad Autónoma Metropolitana, Unidad Iztapalapa, Iztapalapa, Ciudad de México, Mexico.

Methods in Molecular Biology (Clifton, N.J.)

|April 15, 2026

Summary

This summary is machine-generated.

Ex situ orchid conservation utilizes in vitro culture for germplasm preservation. This review covers slow-growth and cryogenic methods, essential for maintaining the valuable Orchidaceae family facing habitat loss.

Keywords:

Cryopreservation Gene stability Orchid fruit Orchid seeds PLBs Plant regeneration Protocorm Slow growth Synthetic seed

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Published on: May 5, 2023

Area of Science:

Horticultural Science
Plant Conservation Biology
Biotechnology

Background:

Orchidaceae family holds significant ecological and economic value.
Over-extraction and habitat destruction threaten orchid populations globally.
Ex situ germplasm preservation is crucial for conservation efforts.

Purpose of the Study:

To review in vitro storage techniques for orchid conservation.
To consolidate findings from 53 studies on orchid germplasm preservation.
To highlight methods for maintaining orchid biodiversity.

Main Methods:

Review of scientific literature on in vitro orchid conservation.
Analysis of studies employing slow-growth techniques for short-term storage.
Evaluation of low-temperature and cryogenic methods for long-term storage.

Main Results:

In vitro culture is effective for ex situ orchid maintenance.
Slow-growth and cryogenic methods offer viable preservation strategies.
Key factors include explant type, culture media, and genetic stability.

Conclusions:

In vitro techniques are vital for the long-term conservation of orchids.
A combination of methods ensures comprehensive germplasm reservoir maintenance.
Further research should focus on optimizing regeneration and genetic integrity.