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Plasmon-Stimulated Colorimetry Biosensor Array for the Identification of Multiple Metabolites
Lin Tian1,2, Ming Cao1, Haorong Cheng1
1School of Materials and Chemical Engineering, Xuzhou University of Technology, Xuzhou 221018, PR China.
ACS Applied Materials & Interfaces
|January 31, 2024
Summary
A novel CuS/ZnS nanozyme exhibits enhanced peroxidase-like activity for detecting multiple metabolites. This plasmon-stimulated biosensor array offers a promising tool for early disease diagnosis and clinical applications.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Analytical Chemistry
Background:
- Developing stable and efficient nanozymes is crucial for metabolite monitoring and early disease diagnosis.
- Nanozymes offer a promising alternative to traditional enzymes due to their stability and cost-effectiveness.
- Metabolite detection plays a vital role in understanding physiological processes and diagnosing diseases.
Purpose of the Study:
- To design and synthesize a novel nanozyme with enhanced peroxidase-like activity for metabolite determination.
- To develop a plasmon-stimulated biosensor array for simultaneous detection and recognition of multiple metabolites.
- To explore the potential of this nanozyme-based biosensor for clinical diagnosis.
Main Methods:
- Hierarchically structured CuS/ZnS nanozyme synthesis.
- Plasmon-stimulated biosensor array fabrication.
- Peroxidase-mimic activity assay using TMB and H2O2.
- Colorimetric detection and principal component analysis (PCA) for metabolite identification.
- Evaluation of detection limits and discrimination of cell lysis.
Main Results:
- The CuS/ZnS nanozyme demonstrated significantly boosted peroxidase-mimic activity under 808 nm laser irradiation due to plasmon-induced hot electron-hole pairs and photothermal effects.
- The biosensor array successfully generated unique colorimetric fingerprints for cysteine, ascorbic acid, and glutathione based on their differential inhibitory effects on nanozyme activity.
- Principal component analysis enabled precise identification of individual metabolites and their mixtures, with a detection limit as low as 1 μM.
- The biosensor assay effectively discriminated cell lysis, indicating its potential for clinical applications.
Conclusions:
- The developed CuS/ZnS nanozyme exhibits excellent peroxidase-mimic capability and stability.
- The plasmon-stimulated biosensor array provides a sensitive and selective platform for multiplexed metabolite detection.
- This approach holds significant promise for non-invasive early disease diagnosis and clinical monitoring.
