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Immunochromatographic visualization detection platform for bitertanol in foods.
Yang Liu1, Lingling Guo1, Xinxin Xu1
1International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
Food Chemistry
|February 4, 2024
Summary
A new gold immunochromatographic assay (GICA) can rapidly detect bitertanol (BIT) fungicide residues in foods. This sensitive method offers reliable on-site monitoring, ensuring food safety by checking for harmful BIT levels.
Area of Science:
- Agricultural Chemistry
- Immunotechnology
- Food Safety Analysis
Background:
- Bitertanol (BIT) is a widely used fungicide in agriculture.
- BIT disrupts hormone regulation and homeostasis, necessitating residue monitoring in food products.
- Existing detection methods can be time-consuming and require complex sample preparation.
Purpose of the Study:
- To develop a novel hapten derivation scheme for preparing a high-affinity anti-BIT monoclonal antibody.
- To establish a visualized gold immunochromatographic assay (GICA) for rapid BIT detection.
- To validate the GICA method for on-site monitoring of BIT residues in food.
Main Methods:
- Designed a hapten derivation scheme based on BIT's chemical structure.
- Prepared a high-affinity, specific, and sensitive anti-BIT monoclonal antibody (IC50 = 4.78 ng/mL).
- Developed a visualized gold immunochromatographic assay (GICA) utilizing antigen-antibody recognition.
Main Results:
- The GICA platform achieved limits of detection of 0.06 mg/kg in cucumber and 0.18 mg/kg in tomato.
- Spiked recoveries ranged from 84.3% to 114.1%, indicating good accuracy.
- GICA results showed no significant difference compared to LC-MS/MS, with reduced sample purification needs.
Conclusions:
- The developed GICA is a reliable, rapid, and sensitive method for detecting BIT residues.
- This assay facilitates on-site, real-time monitoring of BIT in various food matrices.
- The GICA method contributes to enhanced food safety and agricultural practice management.
