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Characterizing Age-related Changes in Intact Mitochondrial Proteoforms in Murine Hearts using Quantitative Top-Down
Andrea Ramirez-Sagredo1, Anju Sunny2, Kellye Cupp-Sutton2
1Oklahoma Medical Research Foundation.
Aging hearts show altered mitochondrial proteoforms. Top-down proteomics revealed changes in protein abundance and post-translational modifications (PTMs) in cardiac aging, offering insights into cardiovascular disease risk.
Area of Science:
- Biochemistry
- Proteomics
- Cardiovascular Science
Background:
- Cardiovascular diseases (CVDs) are a leading cause of mortality, with prevalence increasing with age.
- Cardiac mitochondria are crucial for heart function and highly sensitive to dysfunction.
- The complexity of cardiac mitochondrial proteins and their post-translational modifications (PTMs) presents challenges in understanding CVDs.
Conclusions:
- Top-down proteomics combined with mitochondrial fractionation is effective for analyzing complex cardiac mitochondrial proteomes.
- Age-related alterations in cardiac mitochondrial proteoform abundance and PTMs were successfully detected.
- This approach provides a foundation for understanding mitochondrial dysfunction in cardiac aging and CVDs.
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