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Selective Sampling to and Desorption from Single Solid-Phase Microextraction Arrow Fiber for Replicative and

G Asher Newsome1, Erin R Birdsall1,2, Robert B Cody3

  • 1Smithsonian Institution Museum Conservation Institute, Suitland, Maryland 20746, United States.

Journal of the American Society for Mass Spectrometry
|February 6, 2024
PubMed
Summary
This summary is machine-generated.

A novel interface integrates solid phase microextraction (SPME) with direct analysis in real time (DART) mass spectrometry for rapid, selective chemical analysis. This system allows multiple analyses from a single SPME Arrow, enhancing efficiency and sample differentiation.

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Area of Science:

  • Analytical Chemistry
  • Spectrometry
  • Separation Science

Background:

  • Traditional analytical methods can be time-consuming and require complex sample preparation.
  • Integrating complementary analytical techniques can enhance efficiency and analytical power.

Purpose of the Study:

  • To demonstrate a new analytical interface combining solid phase microextraction (SPME) with direct analysis in real time (DART) mass spectrometry.
  • To showcase the interface's capability for rapid, selective, and multi-point analyses using a single SPME Arrow.

Main Methods:

  • Development of an enclosed interface connecting an SPME device, a DART probe, and a high-resolution mass spectrometer.
  • Utilizing a 20 mm SPME Arrow for multiple discrete DART analyses (up to five) on different fiber areas within one minute.
  • Employing chemometric analysis for distinguishing volatile profiles from rapidly acquired headspace data.

Main Results:

  • Achieved 15% or better center of variance (CV) values for both volatile headspace and direct immersion sampling across multiple runs.
  • Demonstrated selective sampling and differentiation of up to three headspace or five liquid samples from a single SPME Arrow.
  • Successfully constructed a five-point standard addition curve for accurate analyte concentration measurement.

Conclusions:

  • The integrated SPME-DART-MS system offers enhanced analytical functionality with high efficiency and selectivity.
  • The interface enables rapid, multi-point chemical analysis with minimal sample manipulation.
  • This approach provides a versatile platform for analyzing complex samples and determining analyte concentrations accurately.