SYSTEMIC INFLAMMATORY INDICES IN PATIENTS WITH MALIGNANT GLIOMAS AND EFFECTS OF PLATELET SECRETOME IN VITRO.

Area of Science:

• Oncology
• Immunology
• Cell Biology

Background:

• Malignant gliomas (MG) lack effective treatments, driving the search for non-invasive biomarkers.
• Peripheral blood cell ratios and platelet secretome (SPt) are under investigation as potential prognostic indicators for MG.

Purpose of the Study:

• To evaluate peripheral blood cell population ratios in MG patients.
• To investigate the in vitro effect of SPt from MG patients on glioblastoma cell cultures.

Main Methods:

• Peripheral blood samples from MG patients (G2, G3, G4) were analyzed for cell counts.
• The U251 glioblastoma cell line was cultured with SPt from MG patients or SPt pre-incubated with anti-TGF-β1 antibody.
• Mitotic index (MI) was assessed over 72 hours.

Main Results:

• Leukocyte and neutrophil counts, along with neutrophil-to-lymphocyte ratio (NLR) and systemic immune-inflammation index (SII), were significantly increased in MG patients.
• No significant correlation was found between peripheral blood cell counts and tumor malignancy grade.
• SPt from MG patients increased the mitotic index (MI) in U251 cells, correlating with malignancy grade.
• Anti-TGF-β1 antibody partially neutralized the promitotic effect of SPt.

Conclusions:

• Elevated NLR and SII in MG patients lack direct correlation with tumor malignancy.
• Platelet secretome from MG patients exhibits promitotic effects on glioblastoma cells in vitro.
• TGF-β1 may play a role in the promitotic activity of SPt in malignant gliomas.