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Related Concept Videos

Confocal Fluorescence Microscopy01:16

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Related Experiment Video

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Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
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Live Cell Imaging by Single-Shot Common-Path Wide Field-of-View Reflective Digital Holographic Microscope.

Manoj Kumar1,2, Takashi Murata3, Osamu Matoba1,2

  • 1Department of Systems Science, Graduate School of System Informatics, Kobe University, Rokkodai 1-1, Nada, Kobe 657-8501, Japan.

Sensors (Basel, Switzerland)
|February 10, 2024
PubMed
Summary

A new digital holographic microscopy (DHM) system offers wide-field, single-shot, label-free imaging of cells and surfaces. This cost-effective, vibration-insensitive technique enhances biological and micro-surface investigations.

Keywords:
digital holographic microscopylive cell imagingquantitative phase imaging

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Area of Science:

  • Optics and Photonics
  • Biomedical Imaging
  • Microscopy

Background:

  • Quantitative phase imaging (QPI) using digital holographic microscopy (DHM) is a vital label-free technique.
  • DHM excels in analyzing unstained living cells and reflective surfaces.

Purpose of the Study:

  • Introduce a novel wide field-of-view, single-shot, common-path off-axis reflective DHM system.
  • Demonstrate its capabilities for quantitative phase imaging of biological and micro-surface samples.

Main Methods:

  • Developed a compact, stable, and cost-effective common-path off-axis reflective DHM configuration.
  • Utilized a single-shot acquisition for efficient phase imaging.
  • Tested the system with a USAF resolution test target and living plant cells.

Main Results:

  • Achieved high-quality quantitative phase imaging of both reflective and transparent samples.
  • Demonstrated the system's robustness against external vibrations due to its common-path design.
  • Validated the system's suitability for biological specimens and micro-surface metrology.

Conclusions:

  • The proposed DHM configuration provides a versatile, stable, and cost-effective solution for QPI.
  • It offers significant advantages for label-free investigation of biological cells and precise optical metrology of micro-surfaces.