Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Red-shifted excitation enhances the sensitivity of red genetically encoded Ca<sup>2+</sup> indicator and enables crosstalk-free two-photon holographic optophysiology.

Journal of biomedical optics·2025

Same author

Live Cell Imaging by Single-Shot Common-Path Wide Field-of-View Reflective Digital Holographic Microscope.

Sensors (Basel, Switzerland)·2024

Same author

Nonlinear Reconstruction of Images from Patterns Generated by Deterministic or Random Optical Masks-Concepts and Review of Research.

Journal of imaging·2022

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 3, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Dual Field-of-View Off-Axis Spatially Multiplexed Digital Holography Using Fresnel's Bi-Mirror.

Lavlesh Pensia^1,2, Manoj Kumar^3,4, Raj Kumar^1,2

¹CSIR-Central Scientific Instruments Organisation, Sector 30C, Chandigarh 160030, India.

Sensors (Basel, Switzerland)

|February 10, 2024

Summary

This summary is machine-generated.

This study introduces a novel digital holography system using Fresnel

Keywords:

Fresnel bi-mirror Fresnel diffraction method digital holography multiplexed holography

More Related Videos

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

Demonstration of Spin-Multiplexed and Direction-Multiplexed All-Dielectric Visible Metaholograms

Demonstration of Spin-Multiplexed and Direction-Multiplexed All-Dielectric Visible Metaholograms

Published on: September 25, 2020

Related Experiment Videos

Last Updated: Jul 3, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

Demonstration of Spin-Multiplexed and Direction-Multiplexed All-Dielectric Visible Metaholograms

Demonstration of Spin-Multiplexed and Direction-Multiplexed All-Dielectric Visible Metaholograms

Published on: September 25, 2020

Area of Science:

Optics and Photonics
3D Imaging Technologies

Background:

Digital holography (DH) enables 3D imaging by recording amplitude and phase.
Limited field of view (FOV) in DH is constrained by digital sensor pixel pitch.

Purpose of the Study:

To enhance the camera FOV in off-axis digital holography.
To develop a dual FOV DH system using Fresnel's bi-mirror.

Main Methods:

Implemented a novel DH configuration utilizing Fresnel's bi-mirror.
Achieved dual FOV through spatial frequency multiplexing of object beams.
Employed single-shot acquisition and a common-path optical framework.

Main Results:

Demonstrated doubling of the camera FOV compared to existing DH systems.
Successfully imaged two distinct areas of a test object.
Validated the system's accuracy in surface profilometry, matching optical profiler results.

Conclusions:

The proposed dual FOV-DH system effectively expands imaging range.
The simple configuration offers broad applicability in microscopy and optical metrology.