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Related Experiment Video

Updated: Jul 3, 2025

Single-cell Microinjection for Cell Communication Analysis

Single-cell Microinjection for Cell Communication Analysis

Published on: February 26, 2017

Image-activated pico-injection for single-cell analysis.

Zhantao Zhao¹, Heng Zhai¹, Peng Zuo²

¹Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518060, China; Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen, 518060, China.

|February 12, 2024

Summary

This summary is machine-generated.

Image-activated pico-injection (imgPico) enables precise, label-free reagent addition to specific droplets. This technology improves droplet-based assays, like single-cell RNA sequencing, by reducing errors and increasing accuracy.

Keywords:

Label-free detection Pico-injection Real-time analysis Single-cell RNA sequencing

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Area of Science:

Biotechnology
Microfluidics
Genomics

Background:

Droplet-based applications require precise reagent addition for sequential reactions.
Existing pico-injection methods lack specificity, targeting droplets indiscriminately.

Purpose of the Study:

To develop a label-free, on-demand reagent delivery system for droplets.
To enable targeted pico-injection based on real-time image analysis.

Main Methods:

Image-activated pico-injection (imgPico) system developed.
Real-time image analysis algorithms evaluated for droplet detection.
Experimental parameters optimized for imgPico performance.

Main Results:

imgPico successfully injected fluorescent dyes into droplets containing specific cell numbers.
Demonstrated utility in droplet-based single-cell RNA sequencing (scRNA-seq) with high cell density.
Effectively reduced doublets in scRNA-seq data.

Conclusions:

imgPico offers label-free, versatile, and targeted reagent supplementation for droplets.
Represents a significant advancement for droplet-based assays, particularly single-cell analysis.