Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Replisome03:01

The Replisome

33.5K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
33.5K
DNA as a Genetic Template02:05

DNA as a Genetic Template

21.9K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
21.9K
DNA Packaging00:58

DNA Packaging

102.5K
Overview
102.5K
The DNA Replication Fork01:02

The DNA Replication Fork

36.0K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
36.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Programming Dimensional Transitions in DNA Brick Crystals via Interfacial Connectivity.

Angewandte Chemie (International ed. in English)·2026
Same author

Spatial Engineering of Gas Diffusion Layers Overcomes Mass Transport Limitations in Fuel Cells.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Structure-Controlled Molecular Recognition and Charge Transport in Metallized DNA Nanosheets.

Journal of the American Chemical Society·2026
Same author

Author Correction: Atomically precise photothermal nanomachines.

Nature materials·2026
Same author

L-DNA-Based Framework Nucleic Acid Nanodevice for Lysosomal ATP Imaging.

Analytical chemistry·2026
Same author

DNA-Programmed Amorphous PtCu Nanohybrids With Spatially Partitioned Functions for Hydrogen Evolution.

Advanced materials (Deerfield Beach, Fla.)·2026
Same journal

A Ni-Mediated Cross-Coupling Approach to Deuterated <sup>18</sup>F- Fluoromethylated (Hetero)arenes.

Journal of the American Chemical Society·2026
Same journal

Efficient Light-Driven CO<sub>2</sub> Capture and Reversible Release Enabled by Metastable Photoacid-Decorated Metal-Organic Frameworks.

Journal of the American Chemical Society·2026
Same journal

In Situ Raman Spectroscopy Reveals the Dynamic Evolution and Ethanol Dependence of SEI Structure in Li-Mediated N<sub>2</sub> Reduction Reaction.

Journal of the American Chemical Society·2026
Same journal

Solvent Esterification and Stoichiometric Control in Ambient-Grown FAPbI<sub>3</sub> Single-Crystal Solar Cells.

Journal of the American Chemical Society·2026
Same journal

Unlocking Azulene Functionalization via Strain-Induced Azulyne Intermediates.

Journal of the American Chemical Society·2026
Same journal

An Oxazine-Locked Covalent Organic Framework by a Tandem Pinner/Schiff Base Reaction for Hydrogen Peroxide Photosynthesis.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: Jul 3, 2025

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

11.7K

Spacer-Programmed Two-Dimensional DNA Origami Assembly.

Yongjun Liu1, Zheze Dai1, Xiaodong Xie1

  • 1School of Chemistry and Chemical Engineering, New Cornerstone Science Laboratory, Frontiers Science Center for Transformative Molecules, Zhangjiang Institute for Advanced Study and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.

Journal of the American Chemical Society
|February 14, 2024
PubMed
Summary
This summary is machine-generated.

Programming DNA spacer length precisely controls DNA origami assembly. This method enhances hybridization efficiency and pattern diversity, achieving high yields for complex nanostructures.

More Related Videos

Designing a Bio-responsive Robot from DNA Origami
13:32

Designing a Bio-responsive Robot from DNA Origami

Published on: July 8, 2013

22.3K
Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

7.1K

Related Experiment Videos

Last Updated: Jul 3, 2025

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

11.7K
Designing a Bio-responsive Robot from DNA Origami
13:32

Designing a Bio-responsive Robot from DNA Origami

Published on: July 8, 2013

22.3K
Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

7.1K

Area of Science:

  • Nanotechnology
  • Biomolecular Engineering
  • Materials Science

Background:

  • Two-dimensional (2D) DNA origami assembly is a key method for creating advanced 2D materials.
  • DNA spacers are crucial connectors in hybridization-mediated 2D DNA origami assembly, influencing unit binding.

Purpose of the Study:

  • To investigate how programming DNA spacer length affects sticky-end hybridization and the assembly of 2D DNA origami arrays.
  • To optimize the assembly efficiency and pattern diversity of DNA origami nanostructures.

Main Methods:

  • Utilized DNA-PAINT super-resolution imaging to analyze hybridization efficiency.
  • Employed molecular dynamics simulations to study the correlation between spacer length and thermodynamic fluctuations.
  • Programmed DNA spacer lengths to control the binding radius of DNA origami units.

Main Results:

  • Spacer length significantly impacts sticky-end hybridization efficiency in square DNA origami (SDO) unit assembly.
  • Assembly efficiency and pattern diversity are critically dependent on programmed spacer lengths.
  • Achieved high yields (∼98%) for SDO trimer and tetramer assembly using the spacer-programmed strategy.

Conclusions:

  • Spacer length is a critical parameter for precisely controlling 2D DNA origami assembly.
  • This strategy enables the creation of diverse and complex DNA nanostructures with high efficiency.
  • Findings advance the precision assembly of DNA nanostructures for future applications.