Improving the accuracy of nitrogen estimates from nonpoint source in a river catchment with multi-isotope tracers

Climate change can affect precipitation patterns, temperature, and the hydrological cycle, consequently influencing the dynamics of nitrogen (N) within aquatic ecosystems. In this study, multiple stable isotopes (¹⁵N-NO₃/¹⁸O-NO₃ and ²H-H₂O/¹⁸O-H₂O) were used to investigate the N sources and flowpath within the Bogang stream in South Korea. Within the vicinity of the stream with complex land use where various N sources were present, four end-members (rainfall, soil, sewage, and livestock) were sampled and examined. Consequently, spatial-temporal variations of the N sources were observed dependent on the type of land use. During the dry season, sewage accounted for the dominant N source, ranging from 62.2 % to 80.2 %. In contrast, nonpoint sources increased significantly across most sites during the wet season (10.3-41.6 % for soil; 6.3-35.2 % for livestock) compared to the dry season (7.7-28.5 % for soil; 6-13.2 % for livestock). However, sewage (78.7 %) remains dominant, representing the largest ratio at the site downstream of the wastewater treatment plant during the wet season. This ratio showed a notable difference from the calculated N loading ratio of 52.2 %, especially for livestock. This suggests that a significant potential for N legacy effects, given that groundwater flow is likely to be the primary hydrological pathway delivering N to rivers. This study will help to develop water resource management strategies by understanding how the interaction between N sources and hydrological process responds to climate change within sub-basins.