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Updated: Jul 2, 2025
Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
Published on: July 30, 2020
Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging
Michelle S Frei1, Samantha A Sanchez1,2, Longwei Liu2,3,4
1Department of Pharmacology, University of California, San Diego, La Jolla, CA, USA.
None:
Fluorescent biosensors revolutionized biomedical science by enabling the direct measurement of signaling activities in living cells, yet the current technology is limited in resolution and dimensionality. Here, we introduce highly sensitive chemigenetic kinase activity biosensors that combine the genetically encodable self-labeling protein tag HaloTag7 with bright far-red-emitting synthetic fluorophores. This technology enables five-color biosensor multiplexing, 4D activity imaging, and functional super-resolution imaging via stimulated emission depletion (STED) microscopy.
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