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Updated: Jul 2, 2025
10:13
A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
Published on: July 3, 2013
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MiR-378a-5p exerts a radiosensitizing effect on CRC through LRP8/β-catenin axis.
Guolin Hu1, Pengbiao Che2, Ling Deng1
1Department of Oncology, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Qingyuan, China.
Cancer Biology & Therapy
|February 23, 2024
Summary
MicroRNA-378a-5p (miR-378a-5p) enhances colorectal cancer (CRC) radiosensitivity by targeting LRP8 and regulating the β-catenin pathway. Restoring miR-378a-5p may offer a novel therapeutic strategy for overcoming CRC radioresistance.
Area of Science:
- Oncology
- Molecular Biology
- Radiotherapy
Background:
- MicroRNAs (miRNAs) play a crucial role in tumor radiosensitivity.
- The expression of miR-378a-5p is significantly downregulated in colorectal cancer (CRC).
Purpose of the Study:
- To investigate the role of miR-378a-5p in colorectal cancer (CRC).
- To explore the impact of miR-378a-5p on CRC radiosensitivity.
Main Methods:
- Expression analysis of miR-378a-5p in CRC tissues and cell lines.
- Bioinformatics, dual-luciferase reporter assays, and RT-qPCR to identify miR-378a-5p targets.
- In vitro and in vivo experiments assessing CRC cell radiosensitivity following miR-378a-5p modulation and LRP8 targeting.
Main Results:
- Downregulated miR-378a-5p correlates with advanced CRC stage and poor differentiation.
- miR-378a-5p upregulation enhances CRC radiosensitivity by promoting apoptosis and inhibiting proliferation via the LRP8/β-catenin axis.
- Silencing LRP8 (siLRP8) mimics the radiosensitizing effects of miR-378a-5p.
Conclusions:
- miR-378a-5p exhibits a radiosensitizing effect in CRC through the LRP8/β-catenin pathway.
- The miR-378a-5p/LRP8/β-catenin axis represents a potential therapeutic target for overcoming radioresistance in colorectal cancer.
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