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Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids

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Published on: May 12, 2023

Bioorthogonal Reactions in Bioimaging.

Eszter Kozma¹, Péter Kele²

¹Chemical Biology Research Group, Institute of Organic Chemistry, HUN-REN Research Centre for Natural Sciences, Magyar Tudósok Krt. 2, Budapest, 1117, Hungary.

Topics in Current Chemistry (Cham)

|February 24, 2024

Summary

Bioorthogonal chemistry and synthetic biology enable precise labeling of biomolecules for advanced imaging. New probes and minimal tags allow multicolor, super-resolution visualization in live organisms, answering complex biological questions.

Keywords:

Bioorthogonal Fluorescent labeling Fluorogenic probes Live cell imaging

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Area of Science:

Chemical Biology
Molecular Imaging
Synthetic Biology

Background:

Selective labeling of biomolecules is crucial for understanding cellular processes.
Bioorthogonal chemistry and synthetic biology offer powerful tools for site-specific labeling.
Advanced imaging techniques demand novel probes for high-resolution visualization.

Purpose of the Study:

To review recent advancements in bioorthogonal fluorescent probe design for in cellulo/in vivo imaging.
To highlight the role of genetically engineered minimal bioorthogonal tags for protein labeling.
To discuss applications in multicolor and super-resolution imaging of biomolecules.

Main Methods:

Utilizing bioorthogonal chemistry for selective target labeling.
Developing advanced synthetic probes for super-resolution microscopy.
Employing genetically engineered minimal tags for site-specific protein tagging.

Main Results:

Bioorthogonal tools enable selective installation of multiple markers for multicolor/multimodal imaging.
Novel probes facilitate sensitive, low-background imaging at super-resolution in live organisms.
Minimal bioorthogonal tags are crucial for site-specific protein applications.

Conclusions:

The integration of bioorthogonal chemistry, synthetic biology, and advanced probes revolutionizes biomolecular imaging.
These technologies enable unprecedented insights into biological processes at the molecular level.
Future directions focus on enhanced probe design and minimal tag development for complex biological questions.