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Updated: Jul 2, 2025

Super-resolution Imaging of the Bacterial Division Machinery
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Recent advances in super-resolution optical imaging based on aggregation-induced emission.

Feng-Yu Zhu1, Li-Jun Mei1, Rui Tian1

  • 1Wuhan National Laboratory for Optoelectronics, School of Optical and Electronic Information, College of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China. mqzhu@hust.edu.cn.

Chemical Society Reviews
|February 26, 2024
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Summary
This summary is machine-generated.

Aggregation-induced emission (AIE) probes offer enhanced brightness and photostability, revolutionizing super-resolution imaging. This review details AIE mechanisms and their application in advanced microscopy for improved resolution.

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Area of Science:

  • Optics and Photonics
  • Materials Science
  • Biomedical Imaging

Background:

  • Super-resolution imaging requires advanced fluorescent probes with high brightness, contrast, and sensitivity.
  • Traditional fluorescent dyes have limitations in meeting the stringent demands of super-resolution microscopy.
  • Aggregation-induced emission (AIE) offers unique photophysical properties beneficial for imaging.

Purpose of the Study:

  • To review the implementation methods and mechanisms of AIE-based super-resolution imaging.
  • To highlight the advantages of AIE probes in achieving high-resolution imaging.
  • To provide insights into the molecular design of AIEgens for advanced microscopy.

Main Methods:

  • Summarizing progress in AIE-based super-resolution imaging techniques.
  • Analyzing fluorescence switching mechanisms in AIE probes (photochemical, electrostatic, binding-regulated).
  • Discussing the integration of AIE principles with molecular design.

Main Results:

  • AIE probes exhibit high brightness, low background, large Stokes shift, photostability, and biocompatibility.
  • AIE enables spontaneous fluorescence switching, expanding probe selection for super-resolution imaging.
  • Various mechanisms like photochemically-converted, electrostatically controlled, and binding-regulated AIE are effective.

Conclusions:

  • AIE technology significantly enhances super-resolution imaging capabilities.
  • AIE probes are highly promising for next-generation super-resolution microscopy applications.
  • Further molecular design of AIEgens will drive innovation in super-resolution imaging.