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Updated: Aug 9, 2026
Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
Published on: December 26, 2011
Characterization of a glycoprotein fraction with an unusual composition from eel serum
Abstract:
A glycoprotein fraction was extracted from eel serum with a high carbohydrate content of 60% as determined by gas-liquid chromatography. Remarkable is the presence of glucose (5%) which is not attributable to a contaminating glycolipid. Affinity chromatography on wheat germ agglutinin immobilized on to agarose beads results in two glycoproteins with a molecular weight of 17200 and 31300, respectively, as determined by SDS-PAGE (polyacrylamide gel electrophoresis). They differ markedly in their sialic acid content. All of the neuraminic acid is terminally bound and exclusively N-acetylated.
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