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MUC1 promotes RIF by regulating macrophage ROS-SHP2 signaling pathway to up-regulate inflammatory response and

Rongna Liu1,2, Lin Chen1,2, Xin Zhao2

  • 1Department of Obstetrics and Gynecology, Hebei Medical University, Shijiazhuang 050017, China.

Aging
|February 27, 2024
PubMed
Abstract

Insights

Mucin 1 (MUC1) promotes recurrent implantation failure (RIF) by increasing inflammatory responses and inhibiting blood vessel formation. MUC1 may serve as a diagnostic biomarker for RIF.

Area of Science:

  • Reproductive immunology
  • Molecular biology
  • Cell biology

Background:

  • Recurrent implantation failure (RIF) is a complex condition affecting fertility.
  • Understanding the molecular mechanisms underlying RIF is crucial for developing effective diagnostic and therapeutic strategies.

Purpose of the Study:

  • To investigate the role of Mucin 1 (MUC1) in recurrent implantation failure (RIF).
  • To elucidate the molecular mechanisms by which MUC1 influences RIF pathogenesis.

Main Methods:

  • Bioinformatic analysis to identify potential molecular mechanisms.
  • Flow cytometry to quantify macrophage populations (M1 and M2).
  • Immunohistochemistry and Western blotting to assess protein expression.
  • Cell tube-formation assay to evaluate angiogenesis capacity.

Main Results:

  • Bioinformatics suggested MUC1's significant role in RIF.
  • MUC1 overexpression increased M1 macrophages and decreased M2 macrophages.
  • MUC1 inhibited VEGF expression and angiogenesis.
  • MUC1 modulated key proteins in the ROS-SHP2 signaling pathway, including increased P22, P47, gp91, p-TBK1, IFNγ, IL-1β, and decreased p-SHP2, p-PI3K, p-mTOR, HIF1α, VEGF.
  • Inhibitors of ROS and PI3K reversed MUC1's effects.

Conclusions:

  • MUC1 promotes RIF by regulating the macrophage ROS-SHP2 signaling pathway.
  • MUC1 up-regulates inflammatory responses and inhibits angiogenesis, contributing to RIF.
  • MUC1 shows potential as a biomarker for RIF diagnosis.
Keywords:
MUC1ROS-SHP2 signaling pathwayinflammatory responserecurrent implantation failure

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