Advances in mass spectrometry-enabled multiomics at single-cell resolution
Rashmi Kumar1, Kevin J Zemaitis1, James M Fulcher1
1Environmental Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99354, USA.
Current Opinion in Biotechnology
|March 3, 2024
Summary
Single-cell multiomics using mass spectrometry (MS) allows researchers to capture dynamic biological changes. This approach integrates various analytical techniques for comprehensive single-cell analysis.
Area of Science:
- Biochemistry
- Molecular Biology
- Systems Biology
Background:
- Biological systems exhibit complex dynamics across multiple scales.
- Understanding these dynamics requires high-resolution measurements.
- Heterogeneity within biological systems necessitates single-cell analysis.
Purpose of the Study:
- To review mass spectrometry (MS)-enabled multiomic measurements.
- Focus on single-cell or near-single-cell resolution.
- Highlight the importance of capturing dynamic changes in biological systems.
Main Methods:
- Mass spectrometry (MS) as a core analytical technique.
- Coupling MS with other analytical methods.
- Achieving multiomic measurements from individual cells.
Main Results:
- MS offers sensitivity, robustness, and versatility for biomolecule analysis.
- Integration of MS with other techniques enables multiomic profiling.
- Single-cell resolution is crucial for studying biological heterogeneity.
Conclusions:
- MS-enabled multiomics is vital for dissecting complex biological systems.
- This approach provides unprecedented insights into cellular dynamics.
- Future research should leverage these techniques for deeper biological understanding.
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