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Updated: Jul 1, 2025

Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence
Published on: January 17, 2025
Nuclear shape is affected differentially by loss of lamin A, lamin C, or both lamin A and C
Mai Pho1, Yasmin Berrada1, Aachal Gunda1
1Biology Department, University of Massachusetts Amherst, Amherst Center, Massachusetts, United States.
Abstract:
Lamin intermediate filaments form a peripheral meshwork to support nuclear shape and function. Knockout of the LMNA gene that encodes for both lamin A and C results in an abnormally shaped nucleus. To determine the relative contribution of lamin A and C to nuclear shape, we measured nuclear blebbing and circular deviation in separate lamin A and lamin C knockdown and LMNA-/- stable cells. Lamin A knockdown increased nuclear blebbing while loss of lamin A, C, or both increased circular deviation. Overall, loss of lamin A, lamin C or both lamin A/C affect nuclear shape differentially.
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