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Updated: Jul 1, 2025

An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics
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High-Throughput Spectroscopic Analysis of mRNA Capping Level

Chileab Redwood-Sawyerr1, Rochelle Aw1,2, Roberto Di Blasi1

  • 1Department of Chemical Engineering and Imperial College Centre for Synthetic Biology, Imperial College London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 5, 2024
PubMed
Summary

A new biosensor detects mRNA 5' cap structures and 3' polyadenylation sites, crucial for gene expression. This tool assesses mRNA quality and degradation, aiding in transfection experiments.

Keywords:
Biosensing techniquesRNA capRNA isolation and purificationRNA stabilityTranscription

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