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Updated: Jul 1, 2025
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Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates
Published on: September 26, 2013
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Isolation of Human Adipose-Derived Stem Cells
Elizabeth Montano1, Sara Al-Ghadban1, Bruce A Bunnell2
1Department of Microbiology, Immunology and Genetics, School of Biomedical Sciences, University of North Texas Health Sciences Center, Fort Worth, TX, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 13, 2024
Summary
Human adipose-derived stromal/stem cells (hASCs), a type of adult stem cell, are vital for regenerative medicine. This study details laboratory protocols for isolating and expanding these valuable cells from adipose tissue.
Area of Science:
- Regenerative Medicine
- Stem Cell Biology
- Tissue Engineering
Background:
- Human adipose-derived stromal/stem cells (hASCs) represent a significant source of adult stem cells.
- These cells hold considerable potential for diverse applications within regenerative medicine.
Purpose of the Study:
- To present established laboratory protocols for the isolation and expansion of hASCs.
- To provide a reproducible methodology for obtaining clinical-grade hASCs.
Main Methods:
- Isolation of hASCs involves enzymatic digestion of human adipose tissue.
- Subsequent culturing of the digested tissue to expand the isolated stromal/stem cells.
Main Results:
- Successfully isolated and expanded viable hASCs from adipose tissue samples.
- Established a reliable protocol for generating sufficient quantities of hASCs for research.
Conclusions:
- The presented protocols enable efficient isolation and expansion of hASCs.
- These methods support the use of hASCs in regenerative medicine applications.
Keywords:
Adipose-derived stromal/stem cells (ASCs)BiopsyCollagenaseExpansionHumanLipoaspirateMesenchymal stem cells (MSCs)Stromal vascular fraction (SVF)
