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Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion
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Dense, Continuous Membrane Labeling and Expansion Microscopy Visualization of Ultrastructure in Tissues.

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Ultrastructural membrane expansion microscopy (umEXM) enables high-density fluorescent labeling of lipid membranes in intact tissues. This technique achieves nanoscale visualization of membranes and proteins, crucial for understanding cellular structures.

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Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Neuroscience

Background:

  • Lipid membranes are essential for cellular compartmentalization.
  • Visualizing membranes at the nanoscale in intact tissues with high density is challenging.
  • Existing methods lack the resolution and labeling density required for detailed membrane studies.

Conclusions:

  • umEXM provides nanoscale visualization of lipid membranes in intact tissues with high labeling density.
  • The technique enables detailed imaging of cellular structures, including neuronal processes.
  • umEXM offers a pathway towards achieving electron microscopy resolution with light microscopy.