Multifunction fluorescence open source in vivo/in vitro imaging system (openIVIS).

Area of Science:

• Biophotonics
• Open-source hardware and software
• Low-cost scientific instrumentation

Background:

• Open-source microcontrollers and 3D printing enable accessible scientific tools.
• Existing low-cost instruments include microscopes, spectrometers, and sensors.
• These devices support research, education, and experimentation across scientific disciplines.

Purpose of the Study:

• To design and construct a multifunction fluorescent open-source in vivo/in vitro imaging system (openIVIS).
• To integrate a Raspberry Pi, commercial cameras, LEDs, and 3D printed components.
• To enable fluorescent measurements across the visible light spectrum with three excitation wavelengths.

Main Methods:

• Integrated a Raspberry Pi with commercial cameras and LEDs.
• Utilized 3D printed structures and an acrylic housing.
• Developed Python control software for fluorescence measurements.

Main Results:

• The openIVIS system demonstrated performance across diverse experiments, including lab assays and fieldable applications.
• Successfully imaged plants, minerals, and tracked small animal development over ten days.
• Measured a wide dynamic range of fluorescent responses (millimolar to picomolar) across visible wavelengths.

Conclusions:

• Open-source hardware and software, combined with customizable manufacturing, can create versatile, low-cost scientific instruments.
• The openIVIS system offers a flexible and powerful tool for both research and educational settings.
• This study presents a model for developing accessible scientific instrumentation for broader scientific exploration.