Oligonucleotide library screening for identification of virus-specific T-cell receptors

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Marthe-Lina Welters1, Serena Stadler1,2, Vasiliki Anastasopoulou3

  • 1Department of Hematology, Oncology and Tumor Immunology, Charité - Universitätsmedizin Berlin, Berlin, Germany.

European Journal of Immunology
|March 19, 2024
PubMed

We demonstrate an optimized oligonucleotide library-based approach for the identification of virus-reactive T-cell receptors using Epstein-Barr virus as an example. HEK293T served as antigen-presenting cells and were co-cultured with human T cells that were transduced with T-cell receptors in question. T-cell activation was detected by CD137 expression.

Keywords:
Epstein–Barr virusOligonucleotide libraryTCR antigen identificationTarget epitope screeningT‐cell immunology

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