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Enhanced Compound Analysis Using Reactive Paper Spray Mass Spectrometry: Leveraging Schiff Base Reaction for Amino
Marcos Bouza1, Daniel Foest2, Sebastian Brandt2
1Analytical Chemistry Research Group, Department of Physical and Analytical Chemistry, University of Jaén, Campus Las Lagunillas, Jaén 23071, Spain.
Analytical Chemistry
|March 20, 2024
Summary
Reactive paper spray mass spectrometry (PS-MS) enhances amino acid (AA) detection in biofluids. This method optimizes derivatization for faster, more efficient analysis, improving clinical chemistry applications.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Mass Spectrometry
Background:
- Paper spray mass spectrometry (PS-MS) is established for ambient ionization and reaction monitoring.
- Challenges exist in rapid, cost-effective analyte identification in biofluids using PS-MS.
- Lipids and substrate interactions can suppress analyte ionization and impede detection.
Purpose of the Study:
- To develop and optimize reactive PS-MS for enhanced analyte identification in clinical chemistry.
- To leverage the Schiff base reaction for amino acid (AA) derivatization and improve detection efficiency.
- To investigate the impact of reaction conditions and derivatization agents on conversion rates and analyte annotation.
Main Methods:
- Utilized reactive PS-MS with Schiff base (SB) reaction for amino acid (AA) derivatization.
- Optimized reaction parameters including time, temperature, and catalyst (pyridine).
- Evaluated various aromatic aldehydes as derivatization agents and analyzed fragmentation pathways.
Main Results:
- Optimized conditions significantly increased the conversion ratio (CR) of SB formation (e.g., leucine CR from 57% to 89% at 70 °C).
- Specific aromatic aldehyde substituents enhanced CR, and diverse agents provided unique fragmentation data.
- Successfully quantified AAs in human and rat plasma, demonstrating practical application.
Conclusions:
- Reactive PS-MS with optimized Schiff base derivatization offers a rapid and efficient method for challenging analyte detection in biofluids.
- The technique improves ionization efficiency and aids in-depth analyte annotation.
- Shows significant potential for clinical chemistry applications requiring sensitive and cost-effective analysis.
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