Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

10.4K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
10.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Solid-Phase Synthesis of ProTide Fluorogenic Probes Enables Systematic Profiling of Carboxypeptidase Activity.

Journal of the American Chemical Society·2026
Same author

Correction to "Low-Background Cancer Imaging with a Bioorthogonal Fluorescence Probe and Engineered Reporter Enzyme Bearing a Targeting Moiety".

Journal of the American Chemical Society·2026
Same author

Low-Background Cancer Imaging with a Bioorthogonal Fluorescence Probe and Engineered Reporter Enzyme Bearing a Targeting Moiety.

Journal of the American Chemical Society·2026
Same author

Functional Raman Probes for Detecting Enzyme Activities Based on Aggregation Control.

Analytical chemistry·2025
Same author

Near-Infrared Coumarin-Hemicyanine Hybrid Dyes Bearing an Intramolecular Nucleophile for Activatable Fluorescence and Raman Imaging.

Analytical chemistry·2025
Same author

Design of Antigen-Targeting Fluorogenic Probes Utilizing Intramolecular Addition Reaction of Protein-Dye Hybrids.

Journal of the American Chemical Society·2025
Same journal

Synthetic Porous Carbons for High-Energy, High-Power Supercapacitors.

Chemical reviews·2026
Same journal

Navigating Misfolded Terrain: ER-Associated Degradation of Membrane Proteins.

Chemical reviews·2026
Same journal

Ink Design for Printing Perovskite Solar Cells and Modules.

Chemical reviews·2026
Same journal

Advanced Single-Atom Catalysts for Thermal-Catalytic C1 Chemistry.

Chemical reviews·2026
Same journal

Copper-Dependent Polysaccharide Monooxygenases: Mechanism and Function.

Chemical reviews·2026
Same journal

To Biotic or Abiotic: Biohybrid Systems for Artificial Photosynthesis.

Chemical reviews·2026
See all related articles

Related Experiment Video

Updated: Jun 30, 2025

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

12.4K

Activity-Based Fluorescence Diagnostics for Cancer.

Kyohhei Fujita, Yasuteru Urano

    Chemical Reviews
    |March 22, 2024
    PubMed
    Summary
    This summary is machine-generated.

    Activatable fluorescence probes offer high-contrast intraoperative cancer imaging by reacting with cancer-specific enzymes. Recent advances enhance their design and function for improved tumor margin assessment and patient prognosis.

    More Related Videos

    Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
    09:30

    Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

    Published on: January 18, 2017

    12.0K
    Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis
    10:56

    Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis

    Published on: August 1, 2019

    8.2K

    Related Experiment Videos

    Last Updated: Jun 30, 2025

    Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
    12:24

    Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

    Published on: July 17, 2012

    12.4K
    Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
    09:30

    Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

    Published on: January 18, 2017

    12.0K
    Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis
    10:56

    Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis

    Published on: August 1, 2019

    8.2K

    Area of Science:

    • Biomedical Imaging
    • Molecular Diagnostics
    • Surgical Oncology

    Background:

    • Intraoperative assessment of tumor margins is critical for improving cancer surgery outcomes.
    • Molecular fluorescence imaging probes are essential for identifying cancer lesions during surgery.
    • Activatable fluorescence probes offer high-contrast imaging by utilizing cancer-specific enzyme activity.

    Purpose of the Study:

    • To review recent advances in activatable fluorescence probes for cancer imaging.
    • To discuss the design, function, and characteristics of these probes.
    • To explore future prospects in activity-based diagnostics for cancer.

    Main Methods:

    • Review of literature on activatable fluorescence probes over the past two decades.
    • Analysis of probe design, fluorescence control mechanisms, and enzymatic targeting strategies.
    • Discussion of newly identified cancer-specific enzymatic activities.

    Main Results:

    • Development of diverse activatable fluorescence probes with various control mechanisms.
    • Identification of new enzyme biomarkers enabling sensitive and specific cancer visualization.
    • Demonstration of high-contrast imaging potential due to low background and signal amplification.

    Conclusions:

    • Activatable fluorescence probes targeting cancer-specific enzymes show significant promise for intraoperative diagnosis.
    • Continued research in probe design and biomarker discovery will enhance cancer imaging capabilities.
    • Activity-based diagnostics represent a key future direction for cancer detection and treatment.