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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

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Published on: July 25, 2022

Photoswitchable polyynes for multiplexed stimulated Raman scattering microscopy with reversible light control.

Yueli Yang¹, Xueyang Bai¹, Fanghao Hu²

¹Department of Chemistry, MOE Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Tsinghua University, 100084, Beijing, China.

Nature Communications

|March 23, 2024

Summary

Researchers developed Carbow-switch, a novel photoswitchable probe enabling multiplexed stimulated Raman scattering (SRS) imaging in living cells. This breakthrough allows for reversible, light-controlled visualization of cellular dynamics with high precision.

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Area of Science:

Biophysics
Chemical Biology
Optical Microscopy

Background:

Optical imaging is crucial for biological research, with stimulated Raman scattering (SRS) microscopy offering high chemical specificity.
Existing SRS methods lack multiplexing capabilities with reversible photocontrol for dynamic cellular studies.

Purpose of the Study:

To develop a photoswitchable probe for multiplexed SRS microscopy with reversible photocontrol.
To demonstrate advanced imaging of cellular dynamics in living systems.

Main Methods:

Creation of Carbow-switch, a photoswitchable polyyne probe by coupling diarylethene with super-multiplexed Carbow.
Optimization of electronic and vibrational spectroscopy for enhanced photoswitching and SRS response.
Application in reversible, spatial-selective multiplexed SRS imaging of organelles in living cells.

Main Results:

Carbow-switch demonstrated excellent photoswitching under visible light with significant SRS signal enhancement.
Achieved reversible and spatial-selective multiplexed SRS imaging of various organelles in living cells.
Enabled photo-selective time-lapse imaging of organelle dynamics during oxidative stress and protein phase separation.

Conclusions:

Carbow-switch provides a powerful tool for photoswitchable SRS microscopy.
This technology enables new possibilities for studying complex cellular interactions and dynamics with high spatiotemporal precision.