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Pcolce2 overexpression promotes supporting cell reprogramming in the neonatal mouse cochlea
Changling Xu1,2,3, Liyan Zhang4, Yinyi Zhou4
1Health Management Center, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.
Cell Proliferation
|March 26, 2024
Summary
Procollagen C-endopeptidase enhancer 2 (Pcolce2) enables supporting cells to divide and regenerate damaged hair cells. This discovery offers a potential therapeutic target for treating sensorineural hearing loss.
Area of Science:
- Regenerative Medicine
- Otolaryngology
- Cell Biology
Background:
- Hair cell damage causes sensorineural hearing loss.
- Mammalian supporting cells do not regenerate hair cells postnatally.
Purpose of the Study:
- Investigate Procollagen C-endopeptidase enhancer 2 (Pcolce2) as a regulator of supporting cell plasticity.
- Explore Pcolce2's potential for hair cell regeneration.
Main Methods:
- Overexpressed Pcolce2 in supporting cells using adeno-associated virus (AAV)-ie.
- Examined supporting cell cell cycle re-entry in cochlear organoids and postnatal cochlea.
- Assessed hair cell regeneration in a neomycin-induced damage model.
Main Results:
- Adeno-associated virus-Pcolce2 induced supporting cell cell cycle re-entry.
- Regenerated hair cells were observed in the neomycin-damaged model after Pcolce2 overexpression.
- Regenerated hair cells originated from supporting cells that re-entered the cell cycle.
Conclusions:
- Pcolce2 facilitates supporting cell plasticity and cell cycle re-entry.
- Pcolce2 promotes the regeneration of hair cells from supporting cells.
- Pcolce2 represents a promising therapeutic target for hair cell regeneration and hearing loss treatment.
