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Related Concept Videos

Cell Culture01:21

Cell Culture

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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
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Plant Tissue Culture02:57

Plant Tissue Culture

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Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
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Disposable Bioreactors Used in Process Development and Production Processes with Plant Cell and Tissue Cultures.

Rüdiger W Maschke1, Stefan Seidel2, Lia Rossi1

  • 1ZHAW Zurich University of Applied Sciences, School of Life Sciences and Facility Management, Institute of Chemistry and Biotechnology, Wädenswil, Switzerland.

Advances in Biochemical Engineering/Biotechnology
|March 28, 2024
PubMed
Summary

This review examines scalable, disposable bioreactors for plant cell and tissue culture. It highlights wave-mixed, stirred, and shaken bioreactor types, discussing their characteristics and cultivation results for biotechnological production.

Keywords:
Bioreactor selectionEmbryogenic culturesHairy rootsOrbitally shaken cultivation systemsProcess engineering characterizationStirred bioreactorSuspension cellsWave-mixed bag bioreactors

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Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Plant Cell Culture

Background:

  • Bioreactors are crucial for upstream processing in biotechnology.
  • Disposable bioreactors are widely used for medium-scale production of high-value substances.
  • Their design and parameters significantly impact process efficiency and cultivation outcomes.

Purpose of the Study:

  • To review scalable, disposable bioreactors for plant cell and tissue culture.
  • To categorize and present commonly used commercial disposable bioreactor types.
  • To discuss their instrumentation, bioengineering features, cultivation results, and future trends.

Main Methods:

  • Literature review and categorization of disposable bioreactor types.
  • Focus on wave-mixed, stirred, and orbitally shaken bioreactors.
  • Analysis of instrumentation, bioengineering characteristics, and cultivation data.

Main Results:

  • Disposable bioreactors are effective for plant cell and tissue cultivation.
  • Key types include wave-mixed, stirred, and shaken bioreactors.
  • Performance varies based on design, parameters, and cell line.

Conclusions:

  • Scalable, disposable bioreactors are valuable tools in plant biotechnology.
  • Understanding bioreactor types and characteristics is key for optimizing cultivation.
  • Emerging trends point to continued innovation in disposable bioreactor technology.