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Related Experiment Video

Updated: Jun 16, 2026

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device
07:55

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device

Published on: January 26, 2010

Perfusion-Based Antibody Production in the Ambr® 250 Modular.

Vivian Ott1, Jan Ott2, Andry D Mannone3

  • 1ZHAW Zurich University of Applied Sciences, School of Life Sciences and Facility Management, CH-8820 Wädenswil, Switzerland. ottv@zhaw.ch.

Chimia
|May 29, 2025
PubMed
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This study demonstrates novel perfusion processes for monoclonal antibody production using Chinese Hamster Ovary cells in the Ambr 250 Modular system. It achieved ultra-high cell densities and continuous production, paving the way for enhanced biopharmaceutical manufacturing.

Area of Science:

  • Biotechnology
  • Biopharmaceutical Manufacturing
  • Cell Culture Technology

Background:

  • Perfusion mode is crucial for biopharmaceutical production, especially for monoclonal antibodies (mAbs).
  • The Sartorius Ambr 250 Modular system, widely used for mAb process development, was not initially designed for perfusion.
  • Existing perfusion capabilities for the Ambr 250 were limited to its high-throughput version.

Purpose of the Study:

  • To establish perfusion processes in the Ambr 250 Modular system for Chinese Hamster Ovary (CHO) cell culture.
  • To develop an N-1 perfusion process for achieving ultra-high cell densities.
  • To demonstrate continuous mAb production using perfusion in a bench-scale bioreactor.

Main Methods:

  • Utilized the Ambr 250 Modular system combined with Repligen's ATF 1 single-use device.
Keywords:
ATFCHO cellsContinuous perfusionN−1 perfusionScale-down model

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Related Experiment Videos

Last Updated: Jun 16, 2026

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device
07:55

A Multi-Parametric Islet Perifusion System within a Microfluidic Perifusion Device

Published on: January 26, 2010

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06:25

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Published on: July 12, 2024

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  • Developed and tested semi-perfusion and N-1 perfusion strategies.
  • Conducted a 23-day continuous perfusion experiment for mAb production.

    • Main Results:

    • Achieved ultra-high cell densities exceeding 150 x 10^6 cells/mL in the N-1 perfusion process.
    • Successfully demonstrated continuous mAb production over 23 days.
    • Attained a volumetric productivity of 0.65 g/L/day, comparable to larger bioreactor systems.

    Conclusions:

    • The Ambr 250 Modular system, with the ATF 1 device, can be effectively used for perfusion processes.
    • This approach enables the production of high cell densities for subsequent mAb production.
    • The study validates the feasibility of continuous perfusion for efficient mAb manufacturing at the bench scale.