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YAP1/TAZ Mediates Rumen Epithelial Cell Proliferation but Not Short-Chain Fatty Acid Metabolism In Vitro.

Bin Yang^1,2,3, Zebang Xu^2,3, Hongwei Chen^2,3

¹School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China.

Animals : an Open Access Journal From MDPI

|March 28, 2024

Summary

Yes1-associated protein (YAP1) and WW domain-containing transcription regulator protein 1 (TAZ) promote rumen epithelial cell proliferation. However, these key regulators do not influence sodium butyrate-induced rumen development or short-chain fatty acid metabolism.

Keywords:

GA-017 YAP1/TAZ proliferation rumen epithelial cell sodium butyrate verteporfin

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Published on: September 12, 2019

Area of Science:

Ruminant physiology
Molecular biology
Cellular signaling

Background:

Rumen development is crucial for ruminant health and growth.
Yes1-associated protein (YAP1) and WW domain-containing transcription regulator protein 1 (TAZ) are vital for mammalian epithelial regulation.
Understanding YAP1/TAZ roles in rumen epithelium is key for improving animal agriculture.

Purpose of the Study:

To investigate the impact of YAP1/TAZ on rumen epithelial (RE) cell proliferation.
To determine if YAP1/TAZ signaling mediates rumen development induced by sodium butyrate (SB).
To explore the relationship between YAP1/TAZ, Hippo, Wnt, and calcium signaling pathways in RE cells.

Main Methods:

Utilized GA-017 (GA) to activate YAP1/TAZ and verteporfin (VP) to inhibit YAP1/TAZ in RE cells.
Assessed RE cell proliferation and gene expression changes.
Investigated the effects of SB on RE cells with and without YAP1/TAZ modulation.

Main Results:

GA enhanced RE cell proliferation, while VP inhibited it, indicating YAP1/TAZ's role in proliferation.
YAP1/TAZ activation altered Hippo, Wnt, and calcium signaling pathways, increasing CCN1/2 expression.
YAP1/TAZ inhibition decreased BIRC3 expression.
SB treatment promoted RE cell differentiation and short-chain fatty acid (SCFA) metabolism gene expression, independent of YAP1/TAZ activity.

Conclusions:

YAP1/TAZ are potential targets for regulating RE cell proliferation.
YAP1/TAZ do not appear to mediate SB-induced RE development or SCFA metabolism.
SB does not affect YAP1/TAZ activity, suggesting distinct regulatory mechanisms in RE development.