Controllable Thrombolysis Using a Nanobubble-Imaging-Guided rtPA Targeted Delivery Strategy

Area of Science:

• Biomedical Engineering
• Nanomedicine
• Theranostics

Background:

• Thrombotic diseases pose significant health risks, with diagnosis and treatment remaining challenging.
• Nanomedicine offers potential solutions for theranostics but faces hurdles in biosafety, stability, and clinical translation.
• Early diagnosis and timely intervention are critical for managing thrombotic conditions.

Purpose of the Study:

• To design and fabricate a multifunctional nanocarrier for theranostics of thrombotic diseases.
• To combine thrombus-targeted imaging with ultrasound-mediated drug delivery.
• To develop a novel approach for enhanced visualization and treatment of thrombi.

Main Methods:

• Fabrication of S1P@CD-PLGA-rtPA nanobubbles (NBs) integrating SF6-loaded PLGA NBs, CD, S1P, and rtPA.
• Utilizing the specific S1P-S1PR1 interaction for thrombosis targeting and imaging.
• Employing ultrasound to trigger drug release for enhanced thrombolysis.

Main Results:

• S1P@CD-PLGA-rtPA NBs demonstrated rapid and effective thrombosis targeting and imaging capabilities.
• Ultrasound responsiveness enabled accurate and efficient delivery of rtPA to target thrombi.
• Enhanced thrombolysis effectiveness and efficiency were achieved through targeted drug delivery.

Conclusions:

• A novel strategy for targeting thrombi in rats was developed using the S1P-S1PR1 interaction.
• The nanobubbles leverage acoustic properties for combined targeted imaging and ultrasound-mediated drug delivery.
• This approach holds promise for future clinical applications in the targeted diagnosis and treatment of thrombotic diseases.