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Related Experiment Video

Updated: May 13, 2026

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 24, 2014

Sendai virus persistence questions the transient naive reprogramming method for iPSC generation.

Alejandro De Los Angeles¹, Clemens B Hug², Vadim N Gladyshev³

¹McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge MA, USA.

Biorxiv : the Preprint Server for Biology

|April 1, 2024

Summary

Transient naive treatment (TNT) may improve induced pluripotent stem cells (iPSCs), but persistent Sendai virus expression can alter reprogramming factors. Further research is needed for transgene-independent iPSC generation.

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Area of Science:

Stem cell biology
Epigenetics
Developmental biology

Background:

Induced pluripotent stem cells (iPSCs) offer an alternative to embryonic stem cells (ESCs) but exhibit epigenetic and developmental differences.
A recent study proposed transient naive treatment (TNT) to enhance human iPSC (hiPSC) reprogramming and developmental potential.

Approach:

The study re-evaluated the TNT method by analyzing bulk hiPSCs and investigating persistent Sendai virus expression.
Assessed the impact of continuous naive media on Sendai gene expression and reprogramming factors (MYC, SOX2, KLF4).

Key Points:

Sendai virus genes were detected in control pluripotent stem cell (PSC) samples, including human ESCs (hESCs).
Continuous naive media treatment led to elevated Sendai expression and overexpression of exogenous MYC, SOX2, and KLF4.
This altered the expression levels and ratios of key reprogramming factors in hiPSCs.

Conclusions:

The effectiveness of the TNT method requires re-evaluation considering potential confounding factors like persistent viral gene expression.
Future research should focus on delivery methods ensuring rapid elimination of exogenous factors for generating true transgene-independent iPSCs.
Ensuring prompt clearance of viral vectors is crucial for accurate assessment of reprogramming strategies and generating bona fide iPSCs.