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Method Development for Omics Analyses using Schirmer Strips.

Morten Gundersen¹, Per Jensen¹, Christian Nilsen¹

¹Department of Life Sciences and Health, Oslo Metropolitan University, Oslo, Norway.

Current Eye Research

|April 3, 2024

Summary

This summary is machine-generated.

Schirmer strips can be standardized for omics analyses, allowing multiple analyses from one sample. This method accurately measures tear metabolites, even with varying tear volumes or fluorescein dye presence.

Keywords:

Metabolomics dry eye disease methodology omics tear film sampling

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Area of Science:

Ophthalmology
Biochemistry
Analytical Chemistry

Background:

Dry eye disease diagnosis relies on tear analysis.
Standardized methods are needed for omics studies using clinical samples.

Purpose of the Study:

To standardize Schirmer strips for omics analyses.
To enable multiple omics analyses from a single sample.
To assess fluorescein dye's impact on metabolomic analysis.

Main Methods:

Developed a new method for extracting tear metabolites from Schirmer strips.
Collected samples from individuals with normal or reduced tear production.
Analyzed samples using liquid chromatography-mass spectrometry (LC-MS).

Main Results:

Successfully analyzed samples with an average of ~350 features per sample.
The method compensated for varying tear volumes and strip wetting levels.
No bias was observed due to fluorescein dye or different strip wetting levels.

Conclusions:

Longitudinally cut Schirmer strip halves yield numerous metabolomic features.
This method allows for multi-omics analysis from a single sample.
Schirmer strips are suitable for metabolomic analysis across diverse tear volumes and with or without fluorescein.