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Updated: Jun 29, 2025

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
Primer exchange reaction-coupled transcription isothermal amplification as a sensitive biomolecular assay
Jinseo Son1, Seok Hyeon Kim1, Byung Seok Cha1
1Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea. akdong486@konkuk.ac.kr.
Abstract:
We devised a novel strategy that relies on a combination of the primer exchange reaction (PER) with transcription isothermal amplification, termed PER-Trap, for a sensitive biomolecular assay. Its design allowed light-up RNA aptamers to be produced as the final product, leading to the generation of an amplified fluorescence signal. The utility of PER-Trap was successfully demonstrated by the detection of exosomes.
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