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Related Experiment Video

Updated: Jun 29, 2025

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Chemical unclonable functions based on operable random DNA pools.

Anne M Luescher¹, Andreas L Gimpel¹, Wendelin J Stark¹

¹Department of Chemistry and Applied Biosciences, ETH Zürich, Vladimir-Prelog-Weg 1-5, 8093, Zürich, Switzerland.

Nature Communications

|April 5, 2024

Summary

This summary is machine-generated.

Researchers developed a chemical unclonable function (CUF) using DNA to create unique, distributable security tokens. This breakthrough offers a scalable solution for decentralized applications, enhancing object-bound cryptography.

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Area of Science:

Biotechnology and Cryptography
Synthetic Biology for Security Applications

Background:

Physical unclonable functions (PUFs) offer security via unique manufacturing variations but lack distributability, hindering decentralized applications.
Existing PUFs are not suitable for distributed systems due to the non-distributable nature of their unique tokens.

Purpose of the Study:

To develop an unclonable yet distributable function for object-bound cryptography and decentralized applications.
To explore the potential of DNA-based systems for creating novel cryptographic primitives.

Main Methods:

Designed large random DNA pools with segmented structures of alternating constant and random portions.
Utilized these DNA pools to calculate distinct and reproducible outputs from millions of inputs.
Experimentally validated the system with DNA pools exceeding 10^10 unique sequences and over 750 output comparisons.

Main Results:

Demonstrated that segmented random DNA pools can function as chemical unclonable functions (CUFs).
The CUF system exhibits robustness, distributability, and scalability.
Experimental data confirms the ability to generate distinct outputs from millions of inputs reproducibly.

Conclusions:

The proposed chemical unclonable function (CUF) system provides a viable, distributable alternative to traditional PUFs.
CUF technology enables potential applications in anti-counterfeiting, non-fungible objects, and decentralized multi-user authentication.
This proof of concept opens new avenues for secure, object-bound cryptographic solutions in distributed environments.