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Updated: Jun 4, 2026

Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia

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Published on: June 28, 2018

Validation of SeptiCyte RAPID to Discriminate Sepsis from Non-Infectious Systemic Inflammation.

Robert Balk¹, Annette M Esper², Greg S Martin²

¹Rush Medical College and Rush University Medical Center, Chicago, IL 60612, USA.

Journal of Clinical Medicine

|April 9, 2024

Summary

This summary is machine-generated.

The SeptiCyte RAPID molecular test accurately estimates sepsis probability in ICU patients. This clinical validation confirms its utility for differentiating sepsis from systemic inflammation, aiding timely diagnosis and treatment.

Keywords:

SIRS diagnosis host response sepsis sepsis scoring systems

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A Data-Driven Approach to Quantifying Immune States in Sepsis

A Data-Driven Approach to Quantifying Immune States in Sepsis

Published on: February 7, 2025

Area of Science:

Medical Diagnostics
Molecular Biology
Critical Care Medicine

Background:

Sepsis diagnosis remains challenging, often confused with non-infectious systemic inflammation.
Accurate and rapid diagnostic tools are crucial for timely sepsis management in intensive care units (ICUs).

Purpose of the Study:

To clinically validate the SeptiCyte RAPID molecular test for sepsis diagnosis.
To assess the test's ability to discriminate sepsis from systemic inflammation and estimate sepsis probability.

Main Methods:

The study involved clinical validation using retrospective and prospective patient samples from ICUs.
SeptiCyte RAPID, a cartridge-based test, analyzes PAXgene blood RNA for sample-to-answer processing in approximately one hour.
Results were interpreted using Sepsis-2 and Sepsis-3 frameworks, with expert physician adjudication.

Main Results:

SeptiCyte RAPID demonstrated strong performance with Areas Under the ROC Curve (AUCs) ranging from 0.82 to 0.95.
High negative predictive value (0.91) for low-risk sepsis (Band 1) and high positive predictive value (0.81) for high-risk sepsis (Band 4) were observed.
SeptiScore was identified as the most important variable for sepsis diagnosis in multivariable analysis.

Conclusions:

The SeptiCyte RAPID test is validated for estimating sepsis probability in hospitalized ICU patients.
The test performs reliably under both Sepsis-2 and Sepsis-3 diagnostic criteria.
This molecular test aids in differentiating sepsis from systemic inflammation, supporting clinical decision-making.