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Updated: Jun 28, 2025
10:23
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Published on: April 17, 2017
10.1K
The lipid droplet lipidome
Michele Wölk1, Maria Fedorova1
1Center of Membrane Biochemistry and Lipid Research, University Hospital Carl Gustav Carus and Faculty of Medicine of TU Dresden, Germany.
FEBS Letters
|April 11, 2024
Summary
Lipid droplets are dynamic organelles involved in metabolism. This review details their lipidome, exploring lipid classes and molecular species in the core and monolayer, crucial for understanding cellular functions.
Area of Science:
- Cell Biology
- Biochemistry
- Metabolomics
Background:
- Lipid droplets (LDs) are key organelles regulating cellular lipid metabolism and quality control.
- Once viewed as passive storage, LDs are now recognized as dynamic hubs interacting with other organelles.
- While the LD proteome is well-studied, the LD lipidome remains less characterized.
Purpose of the Study:
- To review the current understanding of the lipid droplet lipidome.
- To provide an overview of lipid classes and molecular species within LDs.
- To highlight the importance of characterizing the LD lipidome in health and disease.
Main Methods:
- Literature review of existing research on lipid droplet composition.
- Analysis of studies investigating lipid classes and molecular species in LDs.
- Synthesis of data on the LD lipidome from various physiological and pathological contexts.
Main Results:
- Lipid droplets possess a complex lipidome, including neutral lipids in the core and diverse lipids in the monolayer.
- Specific lipid classes and molecular species within LDs are linked to their regulatory functions.
- Evidence suggests a dynamic interplay between LD lipids and proteins.
Conclusions:
- A comprehensive understanding of the LD lipidome is essential for elucidating LD function.
- Further characterization of the LD lipidome is needed across different cellular states.
- The LD lipidome plays a critical role in cellular metabolic regulation and disease processes.
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